Composite

Part:BBa_K4223008:Design

Designed by: RongKai Tang   Group: iGEM22_HainanU_China   (2022-09-22)


Cas14a1 protein expression system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2555
    Illegal PstI site found at 1345
    Illegal PstI site found at 1511
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2555
    Illegal NheI site found at 2785
    Illegal PstI site found at 1345
    Illegal PstI site found at 1511
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2555
    Illegal BglII site found at 436
    Illegal BamHI site found at 2149
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2555
    Illegal PstI site found at 1345
    Illegal PstI site found at 1511
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2555
    Illegal PstI site found at 1345
    Illegal PstI site found at 1511
    Illegal AgeI site found at 1805
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1


Source

1

References